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KMID : 0359319970370030555
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Korean Journal of Veterinary Research
1997 Volume.37 No. 3 p.555 ~ p.568
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Immunogenicity of outer membrane protein extracted from Salmonella gallinarum in chickens
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Lee Hee-Soo
Kim Sun-Jae
Kim Ki-Seuk
Mo In-Pil
Woo Yong-Ku
Kwon Yong-Kuk
Kim Tae-Jong
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Abstract
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Fowl typhoid caused by Salmonella gallinarum has increased dramatically since 1992 and has caused a great economic losses in chicken industry by characterizing with high mortality. In these studies, we investigated the immunogenicity and protectivity in chickens which were immunized with outer membrane protein(OMP) extracted from isolates of S gallinarum against challenge with live microorganism. Outer membrane proteins were composed of various sizes of molecular weight including 14K, 22K, 31K, 36K, 40K and 55K and the most of them responded strongly against rabbit antisera in immunoblot analysis. The chickens vaccinated with OMP or vaccinated with whole-cell combined with OMP(200㎍/chickens) complex showed higher delayed type hypersensitivity(DTH) response than that of whole-cell vaccinated group. The protective rates of OMP or whole-cell combined with OMP complex group against challenge of S gallinarum were higher (above 75%) than those (45~50%) of whole-cell vaccinated group. All vaccines were safe and the body weight-gains of all vaccinated groups were not significantly different (p<0.05) from those of nonvaccinated control group. In vitro tests, OMP stimulated both the proliferation of lymphocytes and T-lymphocytes, and OMP-induced lymphocyte proliferation was higher in the cells of the immunized chickens with OMP than in those from the control chickens.
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KEYWORD
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Salmonella gallinarum , outer membrane protein , immunogenicity
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